Secondary Metabolites Identification From Lichen Usnea longissima Ach. : Bioactivity Test of Antibacterial

Isolation and identification of secondary metabolite compound from chloroform fraction of Usnea longissima and its bioactivity test as an antibacterial had been conducted. The isolation of the chemical compound was performed by using Gravity Column Chromatography (GCC) and ThinLayered Chromatography (TLC). The result showed the needle crystal shaped isolated compound with translucent white color. The 1D-NMR (H and CNMR) data analysis and comparation of the similar data from the isolated compound literature was (5E, 6E) 5-ethylidene-7-formil-6,7-dihydroxy methyl hept-6-enoate. The antibacterial bioactivity test using the method of paper disc diffusion showed that the chloroform extract inhibited the bacterial growth at the concentration of 100 ppm, 250 ppm, 500 ppm and 1000 ppm for E.coli ATCC 35218, S.auerus ATCC 25923 and S. typhi YCTC. The isolated compound obstructed the bacterial growth at the concentration of 100, 250, 500, and 1000 ppm for E. coli, S. Typhy and it was inactive for S.auerus.